To match the growing demand "in chiral separations": we have established sites in all major world areas involved in pharmaceutical development. DAICEL CHEMICAL INDUSTRIES Ltd in Japan (Arai), CHIRAL TECHNOLOGIES Inc in the USA (Exton), and CHIRAL TECHNOLOGIES EUROPE SAS in France (Illkirch). DAICEL chiral columns are the most widely used and referenced chiral chromatography products in the world. Due to their broad selectivity, good durability and especially high loading capacity, DAICEL Chiral Stationary Phases have become the leading chromatography products for enantiomeric analysis and chiral separations. The Chiral Stationary Phase is the key to achieving lower cost in a chiral separation at a large scale. Technical support is an integral component of the mission of CHIRAL TECHNOLOGIES EUROPE.
DAICEL CHEMICAL INDUSTRIES Ltd DAICEL CHEMICAL INDUSTRIES Ltd is a speciality chemical company with its headquarters in Japan. DAICEL’s major products include organic chemicals, cellulose derivatives, functional products, plastics, aerospace and defence systems. The business unit, at DAICEL, responsible for the production of the chiral chromatography products and related services is the Chiral Pharmaceuticals Ingredients Company (CPI Company). In addition, this unit offers extensive capabilities in biotransformation and a complementary range of specialized pharmaceutical chemicals from DAICEL portfolio that brings value to our customers.
CHIRAL technologies europe
Established in 1995 at Strasbourg (France), CHIRAL TECHNOLOGIES EUROPE is a subsidiary of DAICEL CHEMICALS INDUSTRIES Ltd and is responsible for marketing, sales and technical support of DAICEL chiral separation products, techniques and services in Europe. With the increasing interest in development of single enantiomeric drugs, we are able to help you convert racemic small molecules into single enantiomer chiral products. Equiped with the experience, knowledge and proprietary technologies of DAICEL we offer the pharmaceutical industry, a complete range of products and services for separation of enantiomeric mixtures :
The Chiral Chromatographic Experts Daicel can help you find a chiral phase that meets your project needs. We have the largest portfolio of chiral phases available - including immobilised polysaccharides, coated polysaccharides, proteins, crown ethers and ion exchange columns. They can be used in SFC, SMB and HPLC (Normal, Polar and Reverse phase). As the original developer of polysaccharide phases we have the largest range of coated phases in addition to the new robust immobilised phases. We have phases available in ultra fast 3 µm, high-resolution 5 µm, traditional 10 µm and preparative 20 µm particle sizes. We are sure that we can find a chiral chromatographic separation that will allow you to analyse, isolate or manufacture your chiral compound. To help you with the process of finding the right chiral column we now offer a range of services that are designed to match how you want to work. Screening to Success We offer to screen for free your racemic chiral molecule. Within 5 days of sample receipt we will give you the details of the column you need. For more complex analytical projects we are able to offer a method development service that will develop the method that you need. For a set price of 7,000 Euros we will quickly screen all available phases that match the project requirements. For those customers who have method development capability but are restricted by IP concerns – we can offer a combination of method development guidance in combination with a loan of a set of immobilised chiral columns.
• DAICEL chromatography products for separation of racemic mixtures, the world most widely used and referenced chiral chromatography products • DAICEL chiral HPLC columns • DAICEL bulk Chiral Stationary Phases
• Development of optimal separation chromatography methods for analytical and preparative separation of racemic mixtures • Custom separation services for isolation and purification of enantiomers in quantities ranging from mg to tons • DAICEL technical assistance and support
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www.chiral.fr
CONTENTS
ABOUT CHIRAL TECHNOLOGIES EUROPE p2
CHIRAL HPLC COLUMNS New Immobilised CHIRALPAK® IA, IB, IC New & standard Coated CHIRALPAK® and CHIRALCEL® Reverse phase HPLC columns Accessories for Daicel columns Special applications HPLC columns Preparative columns & bulk Chiral Stationary Phases (CSPs)
p7 p8 p 12 p 26 p 29 p 30 p 35 p 47 p 48 p 49 p 50 p 51 p 53 p 55 p 56 p 56 p 57 p 57 p 59
CHROMTECH CHIRAL HPLC COLUMNS CHIRALPAK® AGP CHIRALPAK® CBH CHIRALPAK® HSA Accessories for CHROMTECH columns
METHOD DEVELOPMENT SERVICE CUSTOM SEPARATION SERVICES Small scale separation Medium scale separation Large scale separation cGMP
TECHNICAL SUPPORT
5
www.chiral.fr
Chiral HPLC columns
chiral hplc columns
DAICEL chiral HPLC columns are the most referenced chiral HPLC columns. Used for their high quality, broad selectivity, durability and high loading capacities, they separate > 99% of all racemic compounds. With the new DAICEL immobilised polysaccharide-derived Chiral Stationary Phase (CSP), the user gains compatibility with all solvents in addition to highly selective Chiral Stationary Phases for enantiomer resolution, which results in an extremely versatile range of CSP’s. As a DAICEL subsidiary we can supply the complete range of Daicel chiral columns for HPLC and SFC applications. The products are available in a range of sizes to meet your application needs whether analytical, semi-preparative or preparative: NEW NEW NEW NEW • CHIRALPAK® IA, CHIRALPAK® IB & CHIRALPAK® IC • CHIRALPAK® IA-3, IB-3 & IC-3 Immobilised stationary phases for increased solvent versatility • CHIRALPAK® & CHIRALCEL® Standard coated polysaccharide stationary phases • CHIRALPAK® AZ-3, AY-3 & CHIRALCEL® OZ-3 Coated stationary phases on our new 3 µm support • CHIRALPAK® AD-3, AS-3 & CHIRALCEL® OD-3, OJ-3 Coated stationary phases on our new 3 µm supports for fast analysis and higher resolution • CHIRALPAK® -R, [-RH] & CHIRALCEL® -R, [-RH] • CHIRALPAK® AD-3R, AS-3R, AY-3R, AZ-3R, & CHIRALCEL® OD-3R, OJ-3R, OZ-3R For reverse phase separation using aqueous-organic mobile phases • DAICEL chiral HPLC columns for special applications For separation of acidic chiral compounds • Preparative columns and bulk stationary phases (CSPs) For larger scale separations
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www.chiral.fr
Chiral HPLC columns
NEW IMMOBILISED CHIRALPAK®
new immobilised chiralpak®
for increased robustness and improved solvent compatibility CHIRALPAK® IA, CHIRALPAK® IB & CHIRALPAK® IC HPLC and SFC columns are packed with NEW Chiral Stationary Phases - consisting of a silica support onto which the polymeric chiral selector (polysaccharide derivatives) has been immobilised. Immobilisation of the polysaccharide derivatives on a matrix is an excellent approach to provide universal solvent compatibility on these highly selective Chiral Stationary Phases. This broadens the range of solvents to be used as mobile phases, thereby introducing new selectivity profiles, improved productivity and robustness in use. CHIRALPAK® IA, CHIRALPAK® IB & CHIRALPAK® IC offer: • High solvent versatility in the selection of the mobile phase composition • Solvent flexibility for the resolution of compounds with limited solubility • High selectivity in the resolution of enantiomers • Robustness and extended durability • Excellent column efficiency • Easy use of the columns NEW CHIRALPAK® IA-3, CHIRALPAK® IB-3 & CHIRALPAK® IC-3 are columns with Chiral selector immobilised on our new 3 µm silica support for: • Fast analysis • Higher resolution • Direct use in most HPLC systems • Direct method transfer between 5 µm and 3 µm • As always, DAICEL high quality Columns are available in a variety of diameters and lengths to meet all your application needs. For larger scale applications, the CSP is also available as a bulk stationary phase (20 µm particle size). Our laboratory evaluation of the immobilised chiral phases show that they are extremely suitable for analytical method development under reverse phase conditions. Many RP and LCMS conditions have been defined using these columns.
www.chiral.fr
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NEW IMMOBILISED CHIRALPAK®
Chiral HPLC columns
NEW IMMOBILISED CHIRALPAK® IA
Reference Product Name Column Length (mm) Internal Diameter (mm)
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) immobilised on A 3 µm & 5 µm silica support Particle Size (µm) Product Type
NEW • C H I R A L P A K ® i A - 3
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
n
80511 80522 80523 80524 80525
CHIRALPAK® IA-3 CHIRALPAK IA-3
®
CH3
CHIRALPAK® IA-3 CHIRALPAK® IA-3 CHIRALPAK® IA-3
silica-gel
H R= N O CH3
• CHIRALPAK® iA
80311 80324 80325
H P L C a n a l y t i c a l c o l u m n s 5 µm
®
CHIRALPAK IA CHIRALPAK® IA CHIRALPAK® IA
10 150 250
4 4,6 4,6
5 5 5
Guard Cartridges (x3) Analytical Analytical
• CHIRALPAK® iA
803C4 80394
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
®
CHIRALPAK® IA CHIRALPAK IA
• CHIRALPAK® iA
80335 80337 80345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALPAK® IA CHIRALPAK® IA CHIRALPAK® IA
• CHIRALPAK® iA
80375 80355
H P L C P R E P ARATIV E c o l u m n s 5 µm
®
CHIRALPAK IA CHIRALPAK® IA
250 250
30 50
5 5
Prep Prep
• CHIRALPAK® iA
80423 80435 80445 80475 80455
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® IA SFC CHIRALPAK® IA SFC CHIRALPAK® IA SFC CHIRALPAK® IA SFC CHIRALPAK® IA SFC
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www.chiral.fr
Chiral HPLC columns
NEW IMMOBILISED CHIRALPAK®
C E L L U L O S E tris ( 3 , 5 - dimeth y lphen y lcarbamate ) immobilised on A 3 µm & 5 µm silica support Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
NEW IMMOBILISED CHIRALPAK® IB
• NEW C H I R A L P A K ® i B - 3
81511 81522 81523 81524 81525
® ®
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3 Guard Cartridges (x3) Analytical
RO OR O
CHIRALPAK® IB-3 CHIRALPAK IB-3 CHIRALPAK IB-3 CHIRALPAK® IB-3 CHIRALPAK® IB-3
O n
Analytical Analytical Analytical
OR
silica-gel
CH3 H R= N O CH3
• CHIRALPAK® iB
81311 81324 81325
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
CHIRALPAK IB CHIRALPAK® IB CHIRALPAK® IB
• CHIRALPAK® iB
813C4 81394
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
CHIRALPAK® IB CHIRALPAK IB
• CHIRALPAK® iB
81335 81337 81345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALPAK® IB CHIRALPAK® IB CHIRALPAK® IB
• CHIRALPAK® iB
81375 81355
®
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALPAK IB CHIRALPAK® IB
• CHIRALPAK® iB
81423 81435 81445 81475 81455
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® IB SFC CHIRALPAK® IB SFC CHIRALPAK® IB SFC CHIRALPAK® IB SFC CHIRALPAK® IB SFC
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NEW IMMOBILISED CHIRALPAK®
Chiral HPLC columns
NEW IMMOBILISED CHIRALPAK® IC
Reference Product Name Column Length (mm) Internal Diameter (mm)
C E L L U L O S E tris ( 3 , 5 - D I C H L O R O P H E N Y L C A R B A M A T E ) immobilised on A 3 µm & 5 µm silica - G E L Particle Size (µm) Product Type
NEW • C H I R A L P A K ® i C - 3
OR O RO OR
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
O n
83511 83522 83523 83524 83525
CHIRALPAK® IC-3 CHIRALPAK IC-3
®
CI
CHIRALPAK® IC-3 CHIRALPAK® IC-3 CHIRALPAK® IC-3
silica-gel
O R= N H CI
• CHIRALPAK® iC
83311 83324 83325
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
CHIRALPAK IC CHIRALPAK® IC CHIRALPAK® IC
• CHIRALPAK® iC
833C4 83394
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
CHIRALPAK® IC CHIRALPAK IC
• CHIRALPAK® iC
83335 83337 83345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALPAK® IC CHIRALPAK® IC CHIRALPAK® IC
• CHIRALPAK® iC
83375 83355
®
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALPAK IC CHIRALPAK® IC
• CHIRALPAK® iC
83423 83435 83445 83475 83455
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® IC SFC CHIRALPAK® IC SFC CHIRALPAK® IC SFC CHIRALPAK® IC SFC CHIRALPAK® IC SFC
11 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
CHIRALPAK® & CHIRALCEL®
DAICEL CHIRALPAK® AD, CHIRALPAK® AS, CHIRALCEL® OD & CHIRALCEL® OJ
The predecessors to the new generation of immobilised columns, traditional CHIRALPAK® & CHIRALCEL® DAICEL coated polysaccharide Chiral Stationary Phases are made with a spherical high quality silica support onto which the polymeric chiral selector (amylose or cellulose derivatives) is physically coated. Due to the coated nature of these chiral supports, solvents should be carefully selected. NEW CHIRALPAK® AD-3, AS-3 & CHIRALCEL® OD-3, OJ-3, are columns with Chiral Stationary Phase coated on our new 3 µm silica support for: • Fast analysis • Higher resolution • Direct use in most HPLC systems • Direct method transfer between 5 µm and 3 µm • As always, DAICEL high quality Analytical and semi-preparative columns for HPLC and SFC applications CHIRALPAK® & CHIRALCEL® chromatography columns are available in a variety of diameters and lengths to meet your application needs. For larger application scales, the CSP is also available as bulk stationary phase (20 µm). Analytical and semi-preparative columns are available with 5 µm and 10 µm particle sizes. The 5 µm columns (-H series) offer higher resolution than the traditional 10 µm columns.
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C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
COATED CHIRALPAK® AD & AD-H
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm)
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on A 3 µm & 5 µm silica support
Product Type
NEW • C H I R A L P A K ® a d - 3
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 150 4 4,6 4,6 4,6 4,6 2,1 3 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical Analytical
n
19511 19522 19523 19524 19525 19594
CHIRALPAK® AD-3 CHIRALPAK AD-3
®
CH3
CHIRALPAK® AD-3 CHIRALPAK® AD-3 CHIRALPAK® AD-3 CHIRALPAK AD-3
®
silica-gel
H R= N O CH3
• CHIRALPAK® ad-H
19311 19324 19325 CHIRALPAK® AD-H CHIRALPAK® AD-H CHIRALPAK® AD-H
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
• CHIRALPAK® ad-H
193C4 19394 CHIRALPAK AD-H
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
CHIRALPAK® AD-H
• CHIRALPAK® ad-H
19335 19337 19345 CHIRALPAK® AD-H CHIRALPAK® AD-H CHIRALPAK® AD-H
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
• CHIRALPAK® ad-H
19375 19355 CHIRALPAK® AD-H CHIRALPAK® AD-H
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
• CHIRALPAK® ad-H
19423 19435 19445 19475 19455
®
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® AD-H SFC CHIRALPAK AD-H SFC CHIRALPAK® AD-H SFC CHIRALPAK® AD-H SFC CHIRALPAK® AD-H SFC
13 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
COATED CHIRALPAK®
AD
Reference
19025
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 1 0 µm silica support
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHIRALPAK® ad
H P L C a n a l y t i c a l c o l u m n 1 0 µm
250 4,6 10 Analytical
RO OR O OR O
CHIRALPAK® AD
n
• CHIRALPAK® AD
19094
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 1 0 µm
150 2,1 10 Analytical
silica-gel
CH3 H R= N O CH3
CHIRALPAK® AD
• CHIRALPAK® AD
19032 19035 19042 19045
H P L C s e m i - p r ep a r a t i v e c o l u m n s 1 0 µm
50 250 50 250 10 10 20 20 10 10 10 10 Guard Semi-Prep Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALPAK® AD CHIRALPAK® AD CHIRALPAK® AD CHIRALPAK® AD
• CHIRALPAK® AD
19145
S F C c o l u m n 1 0 µm
250 20 10 Semi-Prep
CHIRALPAK® AD SFC
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C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
COATED CHIRALPAK® AS & AS-H
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm)
am y lose tris ( ( s ) - α - meth y lben z y L carbamate ) on a 3 µm & 5 µm silica support
Product Type
NEW • C H I R A L P A K ® AS - 3
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
n
20511 20522 20523 20524 20525
CHIRALPAK® AS-3 CHIRALPAK AS-3
®
CH3
CHIRALPAK® AS-3 CHIRALPAK® AS-3 CHIRALPAK® AS-3
silica-gel
H N R= O
*
• C H I R A L P A K ® AS - H 20311 20324 20325 CHIRALPAK AS-H
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
CHIRALPAK® AS-H CHIRALPAK® AS-H
• C H I R A L P A K ® AS - H
203C4 20394 CHIRALPAK® AS-H CHIRALPAK AS-H
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
• C H I R A L P A K ® AS - H
20335 20337 20345 CHIRALPAK® AS-H CHIRALPAK® AS-H CHIRALPAK® AS-H
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
• C H I R A L P A K ® AS - H
20375 20355 CHIRALPAK AS-H
®
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALPAK® AS-H
• C H I R A L P A K ® AS - H
20423 20435 20445 20475 20455
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Semi-Prep Prep
CHIRALPAK® AS-H SFC CHIRALPAK® AS-H SFC CHIRALPAK® AS-H SFC CHIRALPAK® AS-H SFC CHIRALPAK® AS-H SFC
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Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
COATED CHIRALPAK®
AS
Reference
20025
am y lose tris ( ( s ) - α - meth y lben z y L carbamate ) on a 1 0 µm silica support
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• C H I R A L P A K ® AS
H P L C a n a l y t i c a l c o l u m n 1 0 µm
250 4,6 10 Analytical
RO OR O OR O
CHIRALPAK® AS
n
• C H I R A L P A K ® AS
20094
H P L C a n a l y t i c a l S P E C IAL c o l u m n 1 0 µm
150 2,1 10 Analytical
silica-gel
H N R= O CH3
CHIRALPAK® AS
*
• C H I R A L P A K ® AS
20032 20035 20042 20045
H P L C s e m i - p r ep a r a t i v e c o l u m n s 1 0 µm
50 250 50 250 10 10 20 20 10 10 10 10 Guard Semi-Prep Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALPAK® AS CHIRALPAK® AS CHIRALPAK® AS CHIRALPAK® AS
• C H I R A L P A K ® AS
20145
S F C c o l u m n 1 0 µm
250 20 10 Semi-Prep
CHIRALPAK® AS SFC
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C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
COATED CHIRALCEL® OD & OD-H
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm)
C E L L U L O S E tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on A 3 µm & 5 µm silica support
Product Type
NEW • C H I R A L C E L ® O D - 3 H P L C a n a l y t i c a l c o l u m n s 3 µm
OR O RO OR
O n
14511 14522 14523 14524 14525 14594
CHIRALCEL® OD-3 CHIRALCEL OD-3
®
10 50 100 150 250 150
4 4,6 4,6 4,6 4,6 2,1
3 3 3 3 3 3
Guard Cartridges (x3) Analytical Analytical Analytical Analytical Analytical
CH3 H
CHIRALCEL® OD-3 CHIRALCEL® OD-3 CHIRALCEL® OD-3 CHIRALCEL OD-3
®
silica-gel
N O CH3
R=
• CHIRALCEL® OD-H
14311 14324 14325
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
CHIRALCEL® OD-H CHIRALCEL® OD-H CHIRALCEL® OD-H
• CHIRALCEL® OD-H
143C4 14394
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
CHIRALCEL OD-H CHIRALCEL® OD-H
• CHIRALCEL® OD-H
14335 14337 14345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALCEL® OD-H CHIRALCEL® OD-H CHIRALCEL® OD-H
• CHIRALCEL® OD-H
14375 14355
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALCEL® OD-H CHIRALCEL® OD-H
• CHIRALCEL® OD-H
14423 14435 14445 14475 14455
®
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Semi-Prep Prep
CHIRALCEL® OD-H SFC CHIRALCEL OD-H SFC CHIRALCEL® OD-H SFC CHIRALCEL® OD-H SFC CHIRALCEL® OD-H SFC
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Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
COATED CHIRALCEL®
OD
Reference
14025
cellulose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 1 0 µm silica support
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHIRALCEL® OD
H P L C a n a l y t i c a l c o l u m n 1 0 µm
250 4,6 10 Analytical
OR O RO OR
CHIRALCEL® OD
O n
• CHIRALCEL® OD
14032 14035 14042 14045
H P L C s e m i - p r ep a r a t i v e c o l u m n s 1 0 µm
50 250 50 250 10 10 20 20 10 10 10 10 Guard Semi-Prep Semi-Prep Guard Semi-Prep Semi-Prep
silica-gel
CH3 H R= N O CH3
CHIRALCEL® OD CHIRALCEL® OD CHIRALCEL OD
®
CHIRALCEL® OD
• CHIRALCEL® OD
14145
®
S F C c o l u m n 1 0 µm
250 20 10 Semi-Prep
CHIRALCEL OD SFC
www.chiral.fr 18
C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
COATED CHIRALCEL® OJ & OJ-H
cellulose tris ( 4 - meth y lben z oate ) on a 3 µm & 5 µm silica support Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
NEW • C H I R A L C E L ® O J - 3
OR O RO OR
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
O n
17511 17522 17523 17524 17525
CHIRALCEL® OJ-3 CHIRALCEL OJ-3
®
CH3
CHIRALCEL® OJ-3 CHIRALCEL® OJ-3 CHIRALCEL® OJ-3
silica-gel
R= O
• C H I R A L cel ® O J - H
17311 17324 17325
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
CHIRALCEL OJ-H CHIRALCEL® OJ-H CHIRALCEL® OJ-H
• C H I R A L ce l ® O J - H
173C4 17394
®
H P L C a n a l y t i c a l S P E C IAL c o l u m n s 5 µm
150 150 0,3 2,1 5 5 Microflow Analytical
CHIRALCEL® OJ-H CHIRALCEL OJ-H
• C H I R A L ce l ® O J - H
17335 17337 17345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALCEL® OJ-H CHIRALCEL® OJ-H CHIRALCEL® OJ-H
• C H I R A L ce l ® O J - H
17375 17355
®
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALCEL OJ-H CHIRALCEL® OJ-H
• C H I R A L ce l ® O J - H
17423 17435 17445 17475 17455
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Semi-Prep Prep
CHIRALCEL® OJ-H SFC CHIRALCEL® OJ-H SFC CHIRALCEL® OJ-H SFC CHIRALCEL® OJ-H SFC CHIRALCEL® OJ-H SFC
19 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
COATED CHIRALCEL® OJ, OA, OF, OG & OK
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• CHIRALCEL® OJ
17025
H P L C a n a l y t i c a l c o l u m n 1 0 µm
OR
cellulose tris ( 4 - meth y lben z oate ) on a 1 0 µm silica support
CHIRALCEL® OJ 250 4,6 10 Analytical
RO
O
O n
OR
silica-gel
• CHIRALCEL® OJ
17032 17035 17042 17045
®
H P L C s e m i - p r ep a r a t i v e c o l u m n s 1 0 µm
50 250 50 250 10 10 20 20 10 10 10 10 Guard Semi-Prep Semi-Prep Guard Semi-Prep Semi-Prep
R= O
CH3
CHIRALCEL OJ CHIRALCEL® OJ CHIRALCEL OJ
®
CHIRALCEL® OJ
• CHIRALCEL® OJ
17145
S F C c o l u m n 1 0 µm
250 20 10 Semi-Prep
CHIRALCEL® OJ SFC
• C H I R A L C E L ® OA
11022 11025
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
OR
cellulose triacetate on a 1 0 µm silica support
CHIRALCEL® OA CHIRALCEL OA
®
50 250
4,6 4,6
10 10
Guard Analytical
O RO OR
O n
silica-gel
O R= CH3
• CHIRALCEL® oF
15022 15025
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
OR
cellulose T R I S ( 4 - chlorophen y lcarbamate ) on 1 0 µm silica suppor T
CHIRALCEL® OF CHIRALCEL OF
®
50 250
4,6 4,6
10 10
Guard Analytical
O RO OR
O n
silica-gel
H
R=
R=
N O O
C ClH3
• CHIRALCEL
16022 16025
®
oG
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
OR
cellulose T R I S ( 4 - meth y lphen y lcarbamate ) on 1 0 µm silica suppor T
CHIRALCEL® OG CHIRALCEL OG
®
50 250
4,6 4,6
10 10
Guard Analytical
O RO OR
O n
silica-gel
H R= N O CH3
• CHIRALCEL
18022 18025
®
oK
® ®
H P L C a n al y t ical c o l u m n s 1 0 µm
OR
cellulose tricinnamate on a 1 0 µm silica suppor T
CHIRALCEL OK CHIRALCEL OK 50 250 4,6 4,6 10 10 Guard Analytical
RO
O
O n
OR
silica-gel
O R=
www.chiral.fr 20
C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
COATED CHIRALCEL® OB, OB-H, OC & OC-H
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• CHIRALCEL® ob-h
OR O RO OR
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 250 4 4,6 4,6 5 5 5 Guard Cartridges (x3) Analytical Analytical
cellulose triben z oate on a 5 µm & 1 0 µm silica support
O n
12311 12324 12325
CHIRALCEL® OB-H CHIRALCEL® OB-H CHIRALCEL® OB-H
silica-gel
R= O
• CHIRALCEL® ob
12022 12025
®
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
50 250 4,6 4,6 10 10 Guard Analytical
CHIRALCEL OB CHIRALCEL® OB
OR O RO OR
• C H I R A L cel ® O C - H
O n
H P L C a n a l y t i c a l c o l u m n 5 µm
250 4,6 5 Analytical
cellulose T R I S ( phen y lca R bamate ) on a 5 µm & 1 0 µm silica support
13325 CHIRALCEL® OC-H
silica-gel
H N R= O
• CHIRALCEL® oC
13022 13025
®
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
50 250 4,6 4,6 10 10 Guard Analytical
CHIRALCEL OC CHIRALCEL® OC
21 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
NEW COATED CHIRAL COLUMNS
New HPLC and SFC phases that allow effective method development for compounds not fully resolved on other Daicel columns
CHIRALPAK® AY-H, AZ-H & CHIRALCEL® OZ-H HPLC & SFC columns are phases that have been produced for several years by DAICEL. As the selectors are significantly different from existing DAICEL phases they allow new separations to be developed. These phases are now available as packed HPLC and SFC analytical and semi preparative columns. They are effective in developing new compound specific separations. They are an excellent complement in terms of recognition behaviour to the primary screen systems/sets of CHIRALPAK® IA, IB & IC or the alternative CHIRALPAK® AD, AS & CHIRALCEL® OD, OJ. These columns are coated onto the silica support and hence can only be used with mobile phases of alkane/alcohol, acetonitrile/alcohol or mixed alcohol systems. • • • • New recognition profiles that allow new separations Complementary to existing DAICEL screening methodologies Excellent columns efficiency Easy use of the column
www.chiral.fr 22
C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
NEW COATED CHIRALPAK® AZ &
Reference Product Name Column Length (mm) Internal Diameter (mm)
A m y lose tris ( 3 - chloro - 4 - meth y lphen y lcarbamate ) on 3 µm & 5 µm silica - gel .
AZ-H
Product Type
Guard Cartridges (x3) Analytical Analytical Analytical Analytical
Particle Size (µm)
NEW • C H I R A L P A K ® A Z - 3
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3
n
61511 61522 61523 61524 61525
CHIRALPAK® AZ-3 CHIRALPAK AZ-3
®
CI
CHIRALPAK® AZ-3 CHIRALPAK® AZ-3 CHIRALPAK® AZ-3
silica-gel
H R= N O CH3
• CHIRALPAK® AZ-H
61311 61321 61323 61324 61325 CHIRALPAK AZ-H
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 5 5 5 5 5 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
CHIRALPAK® AZ-H CHIRALPAK® AZ-H CHIRALPAK® AZ-H CHIRALPAK AZ-H
®
• CHIRALPAK® AZ-H
61394 CHIRALPAK® AZ-H
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
• CHIRALPAK® AZ-H
61335 61337 61345 CHIRALPAK® AZ-H CHIRALPAK® AZ-H CHIRALPAK® AZ-H
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
• CHIRALPAK® AZ-H
61375 61355 CHIRALPAK® AZ-H CHIRALPAK® AZ-H
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
• CHIRALPAK® AZ-H
61423 61435 61445 61475 61455
®
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® AZ-H SFC CHIRALPAK AZ-H SFC CHIRALPAK® AZ-H SFC CHIRALPAK® AZ-H SFC CHIRALPAK® AZ-H SFC
23 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ®
NEW COATED CHIRALPAK® AY &
Reference Product Name Column Length (mm) Internal Diameter (mm)
A M Y L O S E T R I S ( 5 - C H L O R O - 2 - M E T H Y L P H E N Y L C A R B A M A T E ) on 3 µm & 5 µm silica - G E L
AY-H
Product Type
Guard Cartridges (x3) Analytical
RO OR O
Particle Size (µm)
• NEW C H I R A L P A K ® AY - 3
47511 47522 47523 47524 47525 CHIRALPAK® AY-3 CHIRALPAK AY-3
® ®
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3
CHIRALPAK AY-3 CHIRALPAK® AY-3 CHIRALPAK® AY-3
Analytical Analytical Analytical
OR
O
n
silica-gel
H3C H R= N O CI
• C H I R A L P A K ® AY - H
47311 47321 47323 47324 47325 CHIRALPAK AY-H
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 5 5 5 5 5 Guard Cartridges (x3) Analytical Analytical Analytical Analyrical
CHIRALPAK® AY-H CHIRALPAK® AY-H CHIRALPAK® AY-H CHIRALPAK AY-H
®
• C H I R A L P A K ® AY - H
47394 CHIRALPAK® AY-H
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
• C H I R A L P A K ® AY - H
47335 47337 47345 CHIRALPAK® AY-H CHIRALPAK® AY-H CHIRALPAK® AY-H
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
• C H I R A L P A K ® AY - H
47375 47355 CHIRALPAK® AY-H CHIRALPAK® AY-H
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
• C H I R A L P A K ® AY - H
47423 47435 47445 47475 47455
®
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALPAK® AY-H SFC CHIRALPAK AY-H SFC CHIRALPAK® AY-H SFC CHIRALPAK® AY-H SFC CHIRALPAK® AY-H SFC
www.chiral.fr 24
C H I R A L P A K ® and C H I R A L C E L ®
Chiral HPLC columns
NEW COATED CHIRALCEL® OZ &
C E L L U L O S E tris ( 3 - chloro - 4 - meth y lphen y lcarbamate ) on 3 µm & 5 µm silica - gel .
OZ-H
Product Type
Guard Cartridges (x3) Analytical Analytical Analytical Analytical
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
NEW • C H I R A L C E L ® o Z - 3
OR O RO OR
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 3 3 3 3 3
O n
42511 42522 42523 42524 42525
CHIRALCEL® OZ-3 CHIRALCEL OZ-3
®
CI
CHIRALCEL® OZ-3 CHIRALCEL® OZ-3 CHIRALCEL® OZ-3
silica-gel
H R= N O CH3
• CHIRALCEL® oZ-H
42311 42321 42323 42324 42325 CHIRALCEL OZ-H
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 50 100 150 250 4 4,6 4,6 4,6 4,6 5 5 5 5 5 Guard Cartridges (x3) Analytical Analytical Analytical Analyrical
CHIRALCEL® OZ-H CHIRALCEL® OZ-H CHIRALCEL® OZ-H CHIRALCEL OZ-H
®
• CHIRALCEL® oZ-H
42394
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
CHIRALCEL® OZ-H
• CHIRALCEL® oZ-H
42335 42337 42345
H P L C s e m i - p r ep a r a t i v e c o l u m n s 5 µm
250 20 250 10 10 20 5 5 5 Semi-Prep Guard Semi-Prep Semi-Prep
CHIRALCEL® OZ-H CHIRALCEL® OZ-H CHIRALCEL® OZ-H
• CHIRALCEL® oZ-H
42375 42355
H P L C P R E P ARATIV E c o l u m n s 5 µm
250 250 30 50 5 5 Prep Prep
CHIRALCEL® OZ-H CHIRALCEL® OZ-H
• CHIRALCEL® oZ-H
42423 42435 42445 42475 42455
®
S F C c o l u m n s 5 µm
100 250 250 250 250 4,6 10 20 30 50 5 5 5 5 5 Analytical Semi-Prep Semi-Prep Prep Prep
CHIRALCEL® OZ-H SFC CHIRALCEL OZ-H SFC CHIRALCEL® OZ-H SFC CHIRALCEL® OZ-H SFC CHIRALCEL® OZ-H SFC
25 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ® - R H
CHIRALPAK® -3R & [-RH] & CHIRALCEL® -R & [-RH]
DAICEL REVERSE PHASE HPLC COLUMNS CHIRALPAK® -3R & [-RH] & CHIRALCEL® -R & [-RH] For aqueous-organic mobile phase separation or when pH flexibility is required NEW CHIRALPAK® AD-3R, AS-3R, AZ-3R, AY-3R & CHIRALCEL® OD-3R, OJ-3R, OZ-3R are columns packed with Chiral Stationary Phase coated on our new 3 µm silica support for: • Fast analysis, • Higher resolution, • Direct use in most HPLC systems, • Direct method transfer between 5 µm and 3 µm, • As always, DAICEL high quality. CHIRALPAK® -3R & [-RH] & CHIRALCEL® -R & [-RH] have been developed by DAICEL as the reverse phase versions of CHIRALPAK® AD-H, AS-H, AY-H, AZ-H & CHIRALCEL® OD-H, OJ-H, OZ-H (Columns which have the widest applicability in normal phase mode). They have the same coated chiral selector as found in the normal phase stationary phases, but are coated to a hydrophobic high quality silica support. These reverse-phase columns were developed specifically for aqueous-organic mobile phases. They are suited for applications where the sample is present in aqueous media (e.g. biological samples) or for samples that require flexibility in term of pH range (extreme pH values must be avoided as they can damage the silica gel used in these columns). These columns are also frequently used in LC/MS applications. Analytical columns for reverse phase HPLC CHIRALPAK® -3R & [-RH] & CHIRALCEL® -R & [-RH] chromatography columns are available in a variety of diameters and lengths to meet your application needs. Immobilised chiral phases (CHIRALPAK® IA, CHIRALPAK® IB & CHIRALPAK® IC) show that they are extremely suitable for analytical method development under reverse phase conditions (see column details p. 8). Many RP and LCMS conditions have been defined using these columns.
www.chiral.fr 26
C H I R A L P A K ® and C H I R A L C E L ® - R H
Chiral HPLC columns
REVERSE PHASE CHIRALPAK®
AD-3R, AD[-RH], AS-3R, AS[-RH], AY-3R & AZ-3R
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
NEW • C H I R A L P A K ® A D - 3 R
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 150 4 4,6 4,6 4,6 2,1 3 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical Analytical
re v erse phase t y pe of chiralpak ® ad - 3
19811 19822 19823 19824 19894 CHIRALPAK® AD-3R CHIRALPAK® AD-3R CHIRALPAK® AD-3R CHIRALPAK® AD-3R CHIRALPAK® AD-3R
n
silica-gel
CH3 H R= N O CH3
• C H I R A L P A K ® a d [ - RH ]
19711 19724 CHIRALPAK® AD[-RH] CHIRALPAK® AD[-RH]
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 4 4,6 5 5 Guard Cartridges (x3) Analytical
re v erse phase t y pe of chiralpak ® ad - h
• C H I R A L P A K ® a d [ - RH ]
19794 CHIRALPAK® AD[-RH]
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
re v erse phase t y pe of chiralpak ® ad - h
® NEW • C H I R A L P A K AS - 3 R
OR O RO OR O
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 4 4,6 4,6 4,6 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical
re v erse phase t y pe of chiralpak ® a S - 3
20811 20822 20823 20824 CHIRALPAK® AS-3R CHIRALPAK® AS-3R CHIRALPAK® AS-3R CHIRALPAK® AS-3R
n
CH3
silica-gel
H N R= O
*
• C H I R A L P A K ® a S [ - RH ]
20711 20724 CHIRALPAK® AS[-RH] CHIRALPAK® AS[-RH]
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 4 4,6 5 5 Guard Cartridges (x3) Analytical
re v erse phase t y pe of chiralpak ® a S - h
• C H I R A L P A K ® a S [ - RH ]
20794 CHIRALPAK® AS[-RH]
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
re v erse phase t y pe of chiralpak ® a S - h
NEW • C H I R A L P A K ® AY - 3 R
47811 47822 47823 47825
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 4 4,6 4,6 4,6 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical
re v erse phase t y pe of chiralpak ® aY - 3
CHIRALPAK® AY-3R CHIRALPAK® AY-3R CHIRALPAK® AY-3R CHIRALPAK® AY-3R
NEW • C H I R A L P A K ® A Z - 3 R
61811 61822 61823 61824
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 4 4,6 4,6 4,6 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical
re v erse phase t y pe of chiralpak ® aZ - 3
CHIRALPAK® AZ-3R CHIRALPAK® AZ-3R CHIRALPAK® AZ-3R CHIRALPAK® AZ-3R
27 www.chiral.fr
Chiral HPLC columns
C H I R A L P A K ® and C H I R A L C E L ® - R H
REVERSE PHASE CHIRALCEL® OD-R, OD[-RH], OJ-3R, OJ[-RH] & OZ-3R
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
NEW • C H I R A L C E L ® O D - 3 R
14811 14822 14823 14824 14894 CHIRALCEL® OD-3R CHIRALCEL OD-3R
®
H P L C a n a l y t i c a l c o l u m n s 3 µm
OR
re v erse phase t y pe of chiral C E L ® O D - 3
10 50 100 150 150 4 4,6 4,6 4,6 2,1 3 3 3 3 3 Guard Cartridges (x3)
RO O
O n
Analytical Analytical Analytical Analytical
OR
CHIRALCEL® OD-3R CHIRALCEL® OD-3R CHIRALCEL® OD-3R
silica-gel
CH3 H R= N O CH3
• CHIRALCEL® od[-rh]
14711 14724 CHIRALCEL® OD[-RH] CHIRALCEL OD[-RH]
®
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 4 4,6 5 5 Guard Cartridges (x3) Analytical
re v erse phase t y pe of chiral C E L ® O D - H / O D
• CHIRALCEL® od[-rh]
14794 CHIRALCEL® OD[-RH]
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
re v erse phase t y pe of chiral C E L ® O D - H / O D
• CHIRALCEL® od-r
14611 14625
H P L C a n a l y t i c a l c o l u m n s 1 0 µm
10 250 4 4,6 10 10 Guard Cartridges (x3) Analytical
re v erse phase t y pe of chiral C E L ® O D - H / O D
CHIRALCEL® OD-R CHIRALCEL® OD-R
NEW • C H I R A L C E L ® o j - 3 R
17811 17822 17823 17824 CHIRALCEL® OJ-3R CHIRALCEL® OJ-3R CHIRALCEL® OJ-3R CHIRALCEL® OJ-3R
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 4 4,6 4,6 4,6 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical
RO OR OR O
re v erse phase t y pe of chiral C E L ® O J - 3
O n
silica-gel
R= O CH3
• CHIRALCEL® oj[-rh]
17711 17724 CHIRALCEL® OJ[-RH] CHIRALCEL® OJ[-RH]
H P L C a n a l y t i c a l c o l u m n s 5 µm
10 150 4 4,6 5 5 Guard Cartridges (x3) Analytical
re v erse phase t y pe of chiral C E L ® O J - H
• CHIRALCEL® oj[-rh]
17794
H P L C a n a l y t i c a l S P E C IAL c o l u m n 5 µm
150 2,1 5 Analytical
re v erse phase t y pe of chiral C E L ® O J - H
CHIRALCEL® OJ[-RH]
www.chiral.fr 28
C H I R A L P A K ® and C H I R A L C E L ® - R H
Chiral HPLC columns
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
NEW • C H I R A L C E L ® O Z - 3 R
42811 42822 42823 42824 CHIRALCEL® OZ-3R CHIRALCEL OZ-3R
®
H P L C a n a l y t i c a l c o l u m n s 3 µm
10 50 100 150 4 4,6 4,6 4,6 3 3 3 3 Guard Cartridges (x3) Analytical Analytical Analytical
re v erse phase t y pe of chiral C E L ® O Z - 3
CHIRALCEL® OZ-3R CHIRALCEL® OZ-3R
ACCESSORIES FOR DAICEL COLUMNS
Reference Product Name Column Length (mm) Product Type
00011 00024 00025 CG000D1
Guard cartridge Holder* COLUMN JACKET 1,5 cm COLUMN JACKET 25 cm 150 250
HOLDER FOR GUARD CARTRIDGE HARDWARE HARDWARE COUPLER
Guard COLUMN COUPLER
29 www.chiral.fr
Chiral HPLC columns
S pecial applications H P L C columns
Special applications HPLC columns
Enantioselective HPLC columns for specific applications DAICEL has introduced specific Chiral Stationary Phases to increase the potential for enantiomer separation in special applications : • CHIRALPAK® QD-AX & CHIRALPAK® QN-AX HPLC columns Anion-exchange HPLC columns for separation of chiral acids • CROWNPAK® CR(+) & CROWNPAK® CR(-) HPLC columns Crown ether HPLC columns for separation in acidic mobile phases • CHIRALPAK® WH & CHIRALPAK® MA(+) HPLC columns Ligand-exchange HPLC columns designed for amino-acids separation
Anion Exchange Chiral Stationary Phases
CHIRALPAK® QN-AX & CHIRALPAK® QD-AX are enantioselective weak anion-exchange (AX) HPLC columns. They were developed by Prof. W. Lindner’s group in Vienna and are designed specifically for enantioselective HPLC of chiral acids and possess exceptional enantiomer separation capabilities for acidic chiral compounds containing carboxylic, phosphonic, phosphinic, phosphoric or sulfonic groups. In some cases, weakly acidic compounds such as phenols can also be separated.
www.chiral.fr
30
S pecial applications H P L C columns
Chiral HPLC columns
CHIRALPAK® QD-AX & QN-AX
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• CHIRALPAK® Qd-ax
o - 9 ( tert - but y lcarbamo y l ) q uinidine immobilised on a 5 µm silica support
H
3R
S
4S
silica
31311 31324 31394 31344 31444
CHIRALPAK® QD-AX CHIRALPAK® QD-AX CHIRALPAK® QD-AX CHIRALPAK QD-AX
®
10 150 150 150 150
4 4,6 2,1 20 20
5 5 5 5 5
Guard Cartridges (x3) Analytical Analytical Semi-prep Semi-prep
H N O
O
H
8 9
+ O
N 1S
H O
XO CH
3
N
CHIRALPAK® QD-AX : (8R, 9S) CHIRALPAK® QN-AX : (8S, 9R)
CHIRALPAK® QD-AX SFC
• CHIRALPAK® QN-ax
o - 9 ( tert - but y lcarbamo y l ) q uinine immobilised on a 5 µm silica support
32311 32324 32394 32344 32444 CHIRALPAK® QN-AX CHIRALPAK® QN-AX CHIRALPAK QN-AX
®
10 150 150 150
4 4,6 2,1 20 20
5 5 5 5 5
Guard Cartridges (x3) Analytical Analytical Semi-prep Semi-prep
CHIRALPAK® QN-AX
CHIRALPAK® QN-AX-SFC 150
These two phases are based on two complementary stereoisomeric quinine (QN) and quinidine (QD) derivatives. Due to their pseudo enantiomeric character they usually reveal reversed elution order for opposite enantiomers. They can be used in reversed phase (RP) mode or in polar organic mode (non-aqueous, polar organic solvents containing organic acids and bases as buffer constituents). In addition the separation of chiral basic and neutral compounds may also be possible, but usually normal phase (NP) conditions. In this mobile phase mode, CHIRALPAK® QD-AX & CHIRALPAK® QN-AX behave like a standard Pirkle type Chiral Stationary Phase. They are compatible with all common HPLC solvents (e.g. methanol, acetonitrile, tetrahydrofuran, 1,4-dioxane or chloroform) as well as in a wide pH range spanning from pH 2 to 8. Typical buffers used in hydro-organic mode are acetate, formate, citrate and phosphate.
31 www.chiral.fr
Chiral HPLC columns
S pecial applications H P L C columns
CROWNPAK® CR(+) & CR(-)
Crown Ether Chiral Stationary Phases
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• C R O W N P A K ®C R ( + ) / ( - )
27711 27714 28714 CROWNPAK® CR CROWNPAK CR(+)
®
10 150 150
4 4 4
5 5 5
Guard Analytical Analytical
CROWNPAK® CR(-)
These columns contain a chiral crown ether as a chiral selector, which is coated onto a 5 µm support. Acidic mobile phases such as Perchloric acid pH 1 to 2, are used to operate these columns under standard conditions. Note that to shorten the retention time of hydrophobic samples, the addition of Methanol (15% maximum v/v) has been shown to be effective. These columns are the reference columns for achieving amino acid separations, with the advantage the elution order of the enantiomers can be reversed when necessary (CR(-) column gives the reversed elution order compared to CR(+) column).
www.chiral.fr
32
S pecial applications H P L C columns
Chiral HPLC columns
CHIRALPAK® WH & MA(+)
Ligand Exchange Chiral Stationary Phases
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• CHIRALPAK® WH 25622 CHIRALPAK® WH
25625 CHIRALPAK WH
®
50 250
4,6 4,6
10 10
Guard Analytical
• C H I R A L P A K ® MA ( + ) 21822 CHIRALPAK® MA(+) 50 4,6 3 Analytical
The Chiral Stationary Phases in these columns are made of amino acids and its derivatives coated bonded to silica supports (with a particle size of 10 µm for WH and 3 µm for MA(+)). Since these columns are ligand-exchange type columns, the standard mobile phase to use is an aqueous solution of CuSO4 (0.1 to 2mM). These columns can tolerate organic modifiers such as Methanol and Acetonitrile according specifications in the instruction manual.
33 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
DAICEL 20 µm CHIRALPAK® & CHIRALCEL® Chiral Stationary Phases for large scale enantiomer separatioN
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
DAICEL preparative 20 µm Chiral Bulk Stationary Phases are known for their broad selectivity, durability and high loading capacities. With an excellent batch-to-batch reproducibility, they are particularly adapted to production purposes. All the following Chiral bulk CSPs are directly available from CHIRAL TECHNOLOGIES EUROPE : NEW Immobilised stationary phases compatible with all organic solvent • CHIRALPAK® IA - immobilised amylose support • CHIRALPAK® IC - immobilised cellulose support • CHIRALCEL® OD-I - immobilised cellulose support Standard coated stationary phases • CHIRALPAK® AD - coated amylose support • CHIRALPAK® AS-V - coated amylose support • CHIRALCEL® OD - coated cellulose support • CHIRALCEL® OJ - coated cellulose support • CHIRALPAK® AZ - coated amylose support • CHIRALPAK® AY - coated amylose support • CHIRALCEL® OZ - coated cellulose support • And other cellulose based CSPs for specific applications
Customer support is an integral part of CHIRAL TECHNOLOGIES mission. We provide our preparative scale customers with a free of charge service to identify the optimum stationary phase and mobile phase for the separation of their specific compounds. In addition, to support your preparative method development, we can, upon request, pack analytical columns of various lengths using the matching 20 µm CSPs. These columns are used specifically for developing and optimising methods for preparative processes before scaling up to full-size production. They accurately reflect performance of large preparative columns and the loading data yield accurate projections of the preparative separations. An analytical column packed with the matching 20 µm phase is also available (upon request) to facilitate direct method transfer from analytical to the preparative column.
35 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
CHIRALPAK® ® & CHIRALCEL
DAICEL CHIRALPAK® IA & CHIRALPAK® IC BULK STATIONARY PHASES Immobilised 20 µm amylose tris IA (3,5 dimethylphenylcarbamate stationary phase) & IC (3,5 dichlorophenylcarbamate stationary phase) compatible with all miscible organic solvent and for large scale HPLC and SFC applications CHIRALPAK® IA & CHIRALPAK® IC bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector has been immobilised. Immobilisation of polysaccharide derivatives on a matrix is an excellent approach to implement universal solvent compatibility. This broadens the range of solvents to be used as mobile phases, thereby introducing new selectivity profiles and beneficial CSP characteristics.
www.chiral.fr 36
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
IMMOBILISED CHIRALPAK® IA
OR O RO OR O
& IC
Particle Size (µm) Product Type
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
n
• CHIRALPAK ® IA
CH3
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
silica-gel
H R= N O CH3
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) O N A 2 0 µm silica - G E L
80225 CHIRALPAK® IA
• C H I R A L P A K ® IA
80256 80266
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALPAK® IA CHIRALPAK® IA
• CHIRALPAK® IA
80020 80021
B U L K ST A T I ON A R Y P H A S E S 20 µm
®
CHIRALPAK IA CHIRALPAK® IA
\ \
\ \
20 20
CSP (1 kg) CSP (100 g)
OR O RO OR
O n
• CHIRALpak ® ic
O CI
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
C E L L U L O S E tris ( 3 , 5 - di C H L O R O phen y lcarbamate ) on a 2 0 µm silica - G E L
83225 CHIRALPAK® IC
silica-gel
R= N H CI
• CHIRALPAK® IC
83256 83266
®
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALPAK® IC CHIRALPAK IC
• CHIRALPAK® IC
83020 83021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALPAK® IC CHIRALPAK® IC
37 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
IMMOBILISED CHIRALCEL® od-i
cellulose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 2 0 µm silica support
CHIRALCEL® OD-I bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector : cellulose tris (3,5-dimethylphenylcarbamate) has been immobilised. Immobilisation of polysaccharide derivatives on a matrix is an excellent approach to implement universal solvent compatibility. This broadens the range of solvents to be used as mobile phases, thereby introducing new selectivity profiles and beneficial CSP characteristics recommendation.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
OR
• CHIRALCEL OD-I
®
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
®
O RO OR
O n
82225
CHIRALCEL OD-I
250
4,6
20
Analytical
silica-gel
CH3 H R= N O CH3
• CHIRALCEL® OD-I
82256 82266
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALCEL® OD-I CHIRALCEL® OD-I
• CHIRALCEL® OD-I
82020 82021
®
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL OD-I CHIRALCEL® OD-I
www.chiral.fr
38
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALPAK®
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 2 0 µm silica support
ad
CHIRALPAK® AD bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector : amylose tris (3,5-dimethylphenylcarbamate) has been physically coated. Due to the coated nature of these chiral supports, solvents should be carefully selected.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
OR O RO OR O
• CHIRALPAK ® AD
n
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
CH3
19225
CHIRALPAK® AD
silica-gel
H R= N O CH3
• CHIRALPAK® AD
19256 19266
®
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALPAK AD CHIRALPAK® AD
• CHIRALPAK® AD
19020 19021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALPAK® AD CHIRALPAK® AD
39 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALPAK®
am y lose tris ( ( s ) - α - meth y lben z y lcarbamate ) on a 2 0 µm silica support
as-v
CHIRALPAK® AS-V bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector : amylose tris ((S)-a-methylbenzylcarbamate) has been physically coated. Due to the coated nature of these chiral supports, solvents should be carefully selected.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
OR
• CHIRALPAK ® AS-V
20225
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
O RO OR O
CHIRALPAK® AS-V
n
silica-gel
H N R= O CH3
*
• C H I R A L P A K ® AS-V
20256 20266
®
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALPAK AS-V CHIRALPAK® AS-V
• C H I R A L P A K ® AS-V
20020 20021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALPAK® AS-V CHIRALPAK® AS-V
www.chiral.fr
40
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALCEL®
cellulose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 2 0 µm silica support
OD
Internal Diameter (mm)
CHIRALCEL® OD bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector : cellulose tris (3,5-dimethylphenylcarbamate) has been physically coated. Due to the coated nature of these chiral supports, solvents should be carefully selected.
OR O RO OR
Reference
Product Name
Column Length (mm)
Particle Size (µm)
Product Type
O n
• CHIRALCEL® OD
CH3 H
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
14225
CHIRALCEL® OD
silica-gel
N O CH3
R=
• CHIRALCEL® OD
14256 14266
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALCEL® OD CHIRALCEL® OD
• CHIRALCEL® OD
14020 14021
®
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL® OD CHIRALCEL OD
41 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALCEL®
chiral supports, solvents should be carefully selected.
Reference Product Name Column Length (mm)
cellulose tris ( 4 - meth y lben z oate ) on a 2 0 µm silica support
OJ
CHIRALCEL® OJ bulk stationary phase consists of a 20 µm silica support onto which the polymeric chiral selector : cellulose tris (4-methylbenzoate) has been physically coated. Due to the coated nature of these
Internal Diameter (mm)
Particle Size (µm)
Product Type
OR
• CHIRALCEL® OJ
17225
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
®
O RO OR
O n
CHIRALCEL OJ
250
4,6
20
Analytical
silica-gel
R= O CH3
• CHIRALCEL® OJ
17256 17266
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
CHIRALCEL® OJ CHIRALCEL® OJ
• CHIRALCEL® OJ
17020 17021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
®
CHIRALCEL® OJ CHIRALCEL OJ
www.chiral.fr
42
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALPAK® AY, AZ & CHIRALCEL® OZ
Coated 20 µm cellulose derived bulk stationary phases for large scale HPLC & SFC applications
CHIRALPAK® AY (T304), CHIRALPAK® AZ (50801) & CHIRALCEL® OZ (T405) are HPLC bulk stationary phase that consist of a 20 µm silica support onto which a polymeric chiral selector has been physically coated. Due to the coated nature of the chiral supports, solvents should be carefully selected.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHIRALPAK ® AY PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
OR O RO OR O
A m y lose tris ( 5 - chloro - 2 - meth y lphen y lcarbamate ) on 2 0 µm silica - gel
n
47225
CHIRALPAK® AY
250
4,6
20
Analytical
silica-gel
CI H R= N O CH3
• CHIRALPAK ® AY
47020 47021
BULK STATIONARY PHASES 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALPAK® AY CHIRALPAK® AY
® • CHIRALPAK AZ
OR O RO OR O
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
Analytical
®
A m y lose tris ( 3 - chloro - 4 - meth y lphen y lcarbamate ) on 2 0 µm silica - gel .
n
61225
CHIRALPAK AZ
250
4,6
20
silica-gel
H3C H R= N O CI
• CHIRALPAK ® AZ BULK STATIONARY PHASES 20 µm
61020 61021 CHIRALPAK® AZ CHIRALPAK® AZ \ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
OR O RO OR
• C HIRAL C E L ® O Z
O n
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
C ellulose tris ( 3 - chloro - 4 - meth y lphen y lcarbamate ) on 2 0 µm silica - gel .
CI
42225
CHIRALCEL® OZ
silica-gel
H R= N O CH3
• C HIRAL C E L ® O Z
42020 42021
BULK STATIONARY PHASES 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL® OZ CHIRALCEL® OZ
43 www.chiral.fr
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
COATED CHIRALPAK® ® & CHIRALCEL
Coated 20 µm cellulose derived bulk stationary phases for large scale HPLC & SFC applications CHIRALPAK® T101, CHIRALCEL® OC, CHIRALCEL® OF, CHIRALCEL® OG & CHIRALCEL® OK are HPLC bulk stationary phase that consist of a 20 µm silica support onto which a polymeric chiral selector has been physically coated. Due to the coated nature of the chiral supports, solvents should be carefully selected. To support your preparative method development, we can, upon request, pack analytical columns of various lengths using this 20 µm CSP. It is also available for preparative scale separations in pre-packed columns. In addition, as part of our customer support, we provide those customers buying bulk CSPs, a free of charge service to identify the optimum stationary phase and mobile phase for the separation of their specific compounds.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHIRALPAK ® T101
40225
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
OR O RO OR O
am y lose tris ( 3 , 5 - dimeth y lphen y lcarbamate ) on a 2 0 µm silica support
CHIRALPAK® T101
• CHIRALPAK ® T101
40256 40266
H P L C P R E P ARATIV E C OLUMNS 20 µm
500 500 50 100 20 20 Prep Prep
n
CHIRALPAK® T101 CHIRALPAK® T101
silica-gel
CH3 H R= N O CH3
• CHIRALPAK ® T101
40020 40021
®
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALPAK® T101 CHIRALPAK T101
www.chiral.fr 44
PREPARATIVE COLUMNS & BULK Chiral Stationary Phases (CSPs)
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHIRALCEL® OC
OR O RO OR
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
O n
C E L L U L O S E ( P H E N Y L C A R B A M A T E ) O N A 2 0 µm silica support
H N
13225
CHIRALCEL® OC
silica-gel
R= O
• CHIRALCEL® OC
13020 13021
®
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL OC CHIRALCEL® OC
• C HIRALce l ® O F
OR O RO OR
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
cellulose tris ( 4 - C H L O R O phen y lcarbamate ) on a 2 0 µm silica support
O n
CI
15225
CHIRALCEL® OF
silica-gel
H H N N O O
R= R=
CH Cl 3
• CHIRALCEL® OF
15020 15021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL® OF CHIRALCEL® OF
OR O RO OR
• CHIRALCEL® OG
O n
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
cellulose tris ( 4 - M E T H Y L phen y lcarbamate ) on a 2 0 µm silica support
CI
H H N N O O
16225
CHIRALCEL® OG
silica-gel
CH3 CH3
R= R=
• CHIRALCEL® OG
16020 16021
®
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL OG CHIRALCEL® OG
• CHIRALCEL® OK
OR O RO OR
PREPARATIVE METHOD DEVELOPMENT COLUMN 20 µm
250 4,6 20 Analytical
C E L L U L O S E T R I C I N N A T E O N a 2 0 µm silica support
O n
18225
CHIRALCEL® OK
silica-gel
O R=
• CHIRALCEL® OK
18020 18021
B U L K ST A T I ON A R Y P H A S E S 20 µm
\ \ \ \ 20 20 CSP (1 kg) CSP (100 g)
CHIRALCEL® OK CHIRALCEL® OK
45 www.chiral.fr
C H r OMT E C H - C h i r a l H P L C c o l u m n s
CHROMTECH
chiral hplc columns
These protein-based columns are suitable for several types of compounds; basic compounds can be separated on both CHIRALPAK® AGP and CHIRALPAK® CBH, acidic and neutral compounds can be separated on both CHIRALPAK® AGP and CHIRALPAK® HSA. However, as CHIRALPAK® AGP is a column with an extremely broad applicability, this column should be chosen first, if the analyte has not been separated on any of the columns. There are, however, some types of compounds where one of the other columns may be the first choice : CHIRALPAK® AGP Extremely broad applicability. We believe that this chiral phase as one of the broadest analytical selectivities of any chiral phase. Separates all kinds of compounds : • amines (primary, secondary, tertiary and quartenary) • acids (strong and weak) • nonprotolytes (amides, esters, alcohols) CHIRALPAK® CBH Narrower applicability than CHIRALPAK® AGP. Separates compounds containing one or more basic nitrogens together with one or more basic nitrogens together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester, etc.). CHIRALPAK® HSA Narrower applicability than CHIRALPAK® AGP. Separates preferentially weak and strong acids and non-protolytic compounds.
47 www.chiral.fr
C H r OMT E C h - C h i r a l H P L C c o l u m n s
CHIRALPAK® AGP
CHROMTECH CHIRALPAK® AGP
CHIRALPAK® AGP is the second generation chiral separation column based on the use of α1-acid glycoprotein (AGP) as the CSP. Through a patented process α1-AGP has been immobilized on porous, spherical silica particles (5μm). The surface chemistry of the silica proves a stable chiral separation material with extremely broad applicability. • ENANTIOSELECTIVITY Racemic amines, acids and nonprotolytic compounds can be resolved directly, without derivatization. The column enables resolution of a very large number of chiral compounds from different compound classes. This is due to the unique nature of the CSP, and the fact that enantioselectivity can be induced by choosing a proper mobile phase composition.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHROMTECH CHIRALPAK® AGP COLUMNS 5 µm
30713 30714 30712 30783 30784 30782 30793 30794 30792 30733 30734 30781 30791 30711 CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP CHIRALPAK® AGP (2/pkg) CHIRALPAK® AGP (2/pkg) CHIRALPAK® AGP (2/pkg) 100 150 50 100 150 50 100 150 50 100 150 10 10 10 4 4 4 3 3 3 2 2 2 10 10 3 2 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Semi-Prep Semi-Prep Guard Cartridges (x2) Guard Cartridges (x2) Guard Cartridges (x2)
www.chiral.fr 48
CHIRALPAK® CBH
C H r OMT E C h - C h i r a l H P L C c o l u m n s
CHROMTECH CHIRALPAK® CBH
Cellobiohydrolase (CBH) is a stable enzyme which has been immobilized onto 5 μm spherical silica particles creating the Chiral Stationary Phase in the CHIRALPAK® CBH column. This is also a reverse phase column, used for the direct separation of enantiomers. The column is preferentially used for the separation of the enantiomers of basic drugs from many compound classes.
Reference
Product Name
Column Length (mm)
Internal Diameter (mm)
Particle Size (µm)
Product Type
• CHROMTECH CHIRALPAK® CBH COLUMNS 5 µm
33713 33714 33712 33783 33784 33782 33793 33794 33792 33733 33734 33781 33791 33711 CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH CHIRALPAK® CBH (2/pkg) CHIRALPAK® CBH (2/pkg) CHIRALPAK® CBH (2/pkg) 100 150 50 100 150 50 100 150 50 100 150 10 10 10 4 4 4 3 3 3 2 2 2 10 10 3 2 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Semi-Prep Semi-Prep Guard Cartridges (x2) Guard Cartridges (x2) Guard Cartridges (x2)
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C H r OMT E C h - C h i r a l H P L C c o l u m n s
CHIRALPAK® HSA
CHROMTECH CHIRALPAK® HSA
The chiral selector in this stationary phase is human serum albumin (HSA). The protein has been immobilized onto spherical 5μm particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. The column is operated in the reverse phase mode. With the CHIRALPAK® HSA column, both racemic acids and amino acids can be resolved directly, without derivatization.
Reference Product Name Column Length (mm) Internal Diameter (mm) Particle Size (µm) Product Type
• CHROMTECH CHIRALPAK® HSA COLUMNS 5 µm
34713 34714 34712 34783 34784 34782 34793 34794 34792 34733 34734 34781 34791 34711 CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA CHIRALPAK® HSA (2/pkg) CHIRALPAK® HSA (2/pkg) CHIRALPAK® HSA (2/pkg) 100 150 50 100 150 50 100 150 50 100 150 10 10 10 4 4 4 3 3 3 2 2 2 10 10 3 2 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Analytical Semi-Prep Semi-Prep Guard Cartridges (x2) Guard Cartridges (x2) Guard Cartridges (x2)
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C H r OMT E C h - C h i r a l H P L C c o l u m n s
ACCESSORIES FOR CHROMTECH COLUMNS
Reference
00081 CG000D1 CG000D2
Product Name
GUARD CARTRIDGE HOLDER GUARD COLUMN COUPLER (4 mm) I.D. GUARD COLUMN COUPLER MICRO (3 mm, 2 mm) I.D.
Particle Size (µ)
\ \ \
Product Type
Holder for guard cartridge Coupler Coupler
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m e t h o d de v e l o p m e n t s e r v i ce
method development service
We can provide custom method development for your enantiomer separation. CHIRAL TECHNOLOGIES EUROPE can develop a specific chiral method for the separation of your racemic mixture suited to your analytical, semi-preparative or preparative application. Our team of chromatographers can quickly screen (3 to 4 weeks) all available DAICEL chiral HPLC columns with all possible mobile phase combinations: normal phase, polar phase and reverse phase, depending on your requirement. We respond with the best method to meet the specific project demand. For semi-preparative HPLC custom method development focuses on semi-preparative stationary phases to find the best column possible. Data obtained accurately reflects projections of the preparative separations (milligrams to tens of grams) on 1 cm, 2 cm, and 5 cm I.D. columns. Custom method development can also focus on loading and solubility studies or on particular technical requirements: SFC and Simulated Moving Bed (SMB) systems. Our team of chemist and engineers can also provide custom separation services so that you benefit from the combined strengths of chiral chromatography and DAICEL polysaccharidederived supports.
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c u s t o m s ep a r a t i o n s e r v i ce
custom separation service
For rapid access to pure enantiomers, we can perform your separation for quantities from ca. 100 mg to multi-tons in a cost efficient way.
CHIRAL TECHNOLOGIES EUROPE provides a Custom Separation Service for companies involved in the development of chiral pharmaceutical compounds. Our facilities are equipped with preparative HPLC, preparative SFC and preparative SMB systems; we can provide high purity resolutions of single enantiomers for yields ranging from milligram to 100 kg scale. With CHIRAL TECHNOLOGIES EUROPE, as a fully owned subsidiary of DAICEL, customers have access to the larger scale assets at DAICEL which can be used to separate multi kg to multi tons of racemate. The combined strengths of experienced chiral chromatographers and DAICEL polysaccharidederived supports are the most rapid, efficient and cost-effective way to obtain pure enantiomers. Racemic mixture separation is available at : • Small-scale – mg to g • Medium-scale – g to 10 kg • Large-scale – over 100 kg Experienced in working with the pharmaceutical industry, all our larger separation processes can follow current good manufacturing practises (cGMP).
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c u s t o m s ep a r a t i o n s e r v i ce
Small-scale separation mg to g
Within 10 days, our mg to g separation service provides high yield of both enantiomers for early clinical and toxicology studies. The evaluation is free of charge! The demand for separation of racemic small molecules continues to grow! HPLC and SFC separations is recognised as the fastest method to perform enantioseparation and to provide both enantiomers in ca. 90% yield and to a specification of > 98% enantiomeric excess. With CHIRAL TECHNOLOGIES EUROPE, benefit from the combined strengths of chiral HPLC and DAICEL polysaccharide-derived supports. Let our team of expert scientist and engineers screen all available Daicel chiral HPLC and SFC columns with all possible mobile phase and establish the best protocol for separating your racemic mixture. Once the best protocol is found, take advantage of the preparative HPLC, preparative SFC and SMB systems at our facilities to rapidly provide you with pure enantiomers. This is the most rapid, efficient and cost-effective way to provide the desired enantiomer for further studies.
Medium-scale separation g to kg
We routinely separate g to kg of enantiomers to yield of ca. 90% and a specification of > 98% enantiomeric excess for clinical studies. The evaluation is free of charge! The demand for separation of racemic small molecules continues to grow! HPLC separation is recognised as the fastest method to perform enantioseparation and to provide both enantiomers in ca. 90% yield and to a specification of > 98% enantiomeric excess. By sending a sample to CTE – for no charge you will have access to our team of expert scientist and engineers who will screen all available chiral HPLC columns with all possible mobile phase and established the best protocol for separating your racemic mixture. Once the best method has been found, take advantage of the medium-scale preparative HPLC, preparative SFC and SMB systems at our laboratories to quickly receive g to kg of pure enantiomers of your compound. With CHIRAL TECHNOLOGIES EUROPE, benefit from the combined strengths of chiral HPLC and DAICEL polysaccharide-derived supports to access your desired enantiomer in the fastest and most cost effective way.
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c u s t o m s ep a r a t i o n s e r v i ce
LARGE-scale separation over 100 kg
Get 100 kg to tons of your desired enantiomers API by utilising DAICEL’s chiral chromatography facilities.
For companies involved in the commercialisation of enantiomerically pure drugs, CHIRAL TECHNOLOGIES EUROPE and DAICEL can propose complete outsourcing of the racemic separation step. With us, benefit from the combined strengths of chiral HPLC and DAICEL expertise. This is the- most rapid, efficient and cost-effective way to provide your desired enantiomer in a fast , robust and cost effective way. Our facility at Arai (Japan) is equipped for multi-ton scale separation. In addition to enantioseparation, we can perform upstream or downstream chemical synthesis. You will benefit from DAICEL CHEMICAL INDUSTRY’s expertise in. Used to work with the pharmaceutical industry, all our separation processes follow good laboratory practises (GLP) and can be undertaken to current Good Manufacturing Practice (cGMP) if required.
cGMP
We follow good laboratory practises (GLP) and undertake custom separations to current good manufacturing practises (cGMP)
Working predominantly for the pharmaceutical companies, CHIRAL TECHNOLOGIES EUROPE and DAICEL take extreme care to follow updated good laboratory practises (GLP). This is a guarantee for our customers that they can trust our method development activities. In addition our custom separation services can undertake separations to current Good Manufacturing Practice (cGMP).
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T E C HNI C AL SU P P ORT
TECHNICAL SUPPORT
CHIRAL TECHNOLOGIES EUROPE Benefit from DAICEL technical support Our Technical Support Team as well as our chromatography experts, scientist and engineers will assist you with your questions related to the use of our analytical columns, preparative separations columns and issues associated with bulk CSPs for large scale applications. Our team of chromatographers and engineers can also develop custom method and protocol to separate your specific enantiomer or provide custom separation services so that you benefit from the combined strengths of chiral chromatography and DAICEL polysaccharide-derived supports. Contact us at : support@chiral.fr Our network :
North / South America Chiral Technologies
Europe CHIRAL TECHNOLOGIES EUROPE Japan / Korea Daicel Chemical Industries China Daicel Chiral Technologies
India Daicel Chiral Technologies
North/South America C iral Technologies, Inc. h 800 North Five Points Road, West Chester PA 19380, USA Tel. : +1-610-594-2100 Fax : +1-610-594-2325 www.chiraltech.com chiral@chiraltech.com
Europe C iral Technologies Europe h Parc d'Innovation Bd Gonthier d'Andernach 67400 Illkirch Cedex, France Tél. : +33-388-795-200 Fax : +33-388-667-166 www.chiral.fr cte@chiral.fr
India Daicel Chiral Technologies, Pvt, Ltd Lab No. 4A, Phase III, ICICI Knowledge Park, Genome Valley, Turkapally, Shameerpet, Ranga Reddy Dist Hyderabad-500 078, A.P., India Tel. : +91-40-23480103 +91-40-23480134 Fax : +91-40-23480104 chiral@daicel-india.com
China Daicel Chiral Technologies Co., Ltd. Part C, FL5, No.16, Xiya Road No. 69 Waigaoqiao Free Trade Zone, Shanghai, 200131, China Tel. : +86-21-50460086 Fax : +86-21-50462321 www.daicelchiraltech.cn chiral@daicelchiraltech.cn
Japan / Korea Daicel Chemical Industries, Ltd. CPI Company JR Shinagawa East Bldg, 2-18-1 Konan, Minato-ku Tokyo 108-8230, Japan Tel. : +81-3-6711-8222 Fax : +81-3-6711-8228 www.daicel.co.jp chiral@daicel.co.jp
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T E C HNI C AL SU P P ORT
daicel quality
To be sure of the quality of your racemic separation, rely on DAICEL quality, the most widely used and referenced chiral separation products worldwide.
CHIRAL TECHNOLOGIES EUROPE is a wholly owned subsidiary of DAICEL, established to market and sell Daicel chiral separation products and services in Europe. Our products are the most widely used and reference products worldwide for chiral separation thanks to the unequalled quality of DAICEL proprietary technologies. Broad selectivity, durability and high loading capacity ensure that DAICEL CSP are the leading chromatography product for enantiomeric analysis and chiral separation. Their outstanding capabilities provide you with a powerful tool. All our products have excellent standard of quality, reproducibility and an excellent batch to batch reproducibility. They are all individually tested and the results of the tests are provided with the product you receive. For detailed description of the product sold by CHIRAL TECHNOLOGIES EUROPE, please refer to our product section. A team of expert chromatographers can develop methods, provide technical support and develop new applications. Our products and custom separation services are accomplished in facilities that comply with cGMP quality guidelines.
APPLICATION GUIDE
A fully searchable database with over 700 examples to help you find the most appropriate DAICEL product and chromatography method
To assist our customers in finding the most appropriate DAICEL CSP for an enantiomer separation, CHIRAL TECHNOLOGIES EUROPE has developed an application guide. Available on CD-ROM, it contains over 700 examples, the majority of which have therapeutic properties. This guide is organised in a database that allow the user to search by compound name, compound structure, DAICEL column name, mobile phase or therapeutic indication. Regularly updated, this application guide is intended to be intuitive, straightforward and efficient.
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